Biological Activity
Taurodeoxycholic acid-d4 (TDCA-d4) is intended for use as an internal standard for the quantification of taurodeoxycholic acid by GC- or LC-MS. TDCA is a taurine-conjugated form of the secondary bile acid deoxycholic acid .1 TDCA stimulates chloride ion secretion through calcium-activated chloride ion channels and cystic fibrosis transmembrane conductance regulator (CFTR) in Calu-3 airway epithelial cell monolayers when applied basolaterally.2 Serum levels of taurodeoxycholic acid increase approximately 5-fold within two hours during an oral lipid tolerance test in humans.1
TDCA-d4 MaxSpec? standard is a quantitative grade standard of TDCA-d4 that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. The verified concentration is provided on the certificate of analysis. This TDCA-d4 MaxSpec? standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.
References
1.Schmid, A., Neumann, H., Karrasch, T., et al.Bile acid metabolome after an oral lipid tolerance test by liquid chromatography-tandem mass spectrometry (LC-MS/MS)PLoS One11(2)e0148869(2016)
2.Hendrick, S.M., Mroz, M.S., Greene, C.M., et al.Bile acids stimulate chloride secretion through CFTR and calcium-activated Cl- channels in Calu-3 airway epithelial cellsAm. J. Physiol. Lung. Cell. Mol. Physiol.307(5)L407-L418(2014)
