Description
Geldanamycin-biotin (1320265-38-0) is a useful biotinylated ligand for affinity purification of HSP-90 and HSP-90- dependent client proteins.
Uses
Geldanamycin-biotin is a biotinylated ligand for affinity purification of HSP-90 and HSP-90-dependent proteins.
Biochem/physiol Actions
Geldanamycin-Biotin is a benzoquinone ansamycin antitumor antibiotic. Geldanamycin binds specifically to Hsp90 (HeatShock Protein 90). and to its endoplasmic reticulum homologue GP96. The Hsp90 chaperone is required for the activation of several families of eukaryotic protein kinases and nuclear hormone receptors, many of which are proto-oncogenic and play a prominent role in cancer. The geldanamycin antibiotic has antiproliferative and antitumor effects, as it binds to Hsp90, inhibits the HSP90-mediated conformational maturation/refolding reaction, and results in the degradation of Hsp90 substrates. Hsp90 also plays a key role in regulating the physiology of cells exposed to environmental stress and thus, geldanamycin interferes with cellular stess response.
References
1) Clevenger?et al.?(2004),?Biotinylated geldanamycin; J. Org. Chem.,?69?4375
