PPA is the product of peroxidase-catalyzed reduction of PPHP. It can be used as a reference standard for HPLC analysis of peroxidase assays.
Uses
5-Phenyl-4E-pentenol is a product of the peroxidase-catalyzed reduction of PPHP. 5-Phenyl-4E-pentenol can be rapidly isolated by solid phase extraction and quantified by isocratic reversed-phase high performance liquid chromatography (HPLC) with UV detection. 5-Phenyl-4E-pentenol is a fluorescent dye that can be used to determine the kinetic properties of heme-containing and non-heme peroxidases and to evaluate the ability of oxidative compounds to serve as substrates for peroxidase reduction.[1].
[1] Weller P E, et al. Enzymatic reduction of 5-phenyl-4-pentenyl-hydroperoxide: detection of peroxidases and identification of peroxidase reducing substrates[J]. Archives of biochemistry and biophysics, 1985, 243(2): 633-643. DOI:10.1016/0003-9861(85)90541-7
