126824-24-6

beige powder

Suitable as Ca2+-indicator (fluorescence suppressed by Ca2+). Chelator with a greater selectivity for calcium compared to magnesium.

Ca²⁺-chelator exhibiting a 10⁵-fold greater affinity for Ca²⁺ (110 nM) than for Mg²⁺. After BAPTA complexes with Ca²⁺, the UV spectrum shifts from 254 to 279 nm at pH >6.0. Useful for spectrophotometric monitoring of extracellular Ca²⁺ levels, especially transient phenomena, because of its insensitivity to pH. Has been used for the measurement and control of Ca²⁺ levels in eggs, chromaffin cells, muscle cells, retinal cells, and pancreatic cells. In conjunction with certain formazan dyes, BAPTA can be used in a colorimetric assay for Mg²⁺. Has neuroprotective effects in vitro against excitotoxic damage and spontaneous recurrent seizures, such as epilepsy.