Uses
COTI 2, efficiently inhibits the proliferation rate of all the tested cell lines following 72 h of treatment. COTI-2 is significantly effective at inhibiting tumor cell proliferation in all three cell lines (COLO-205, HCT-15, and SW620).
Biological Activity
coti-2 activates mutant forms of p53.p53 has varoius mechanisms of anticancer function and plays a critical role in genomic stability, apoptosis and inhibition of angiogenesis.
in vitro
previous study tested the efficacy of coti-2 against a diverse group of human cancer cell lines with different genetic mutation backgrounds. results showed that coti-2 efficiently inhibited the proliferation rate of all the tested cell lines following 72 h of treatment. most cell lines showed nanomolar sensitivity to coti-2 treatment. in addition, coti-2 was significantly more effective at inhibiting tumor cell proliferation than either cetuximab or erlotinib in colo-205, hct-15, and sw620 cell lines. notably, all three lines were insensitive to growth inhibition to any degree in response to low concentrations of cetuximab and erlotinib, but highly sensitive to even low doses of coti-2 [1].
in vivo
the effects of coti-2 on inhibiting the growth of ht-29 and shp-77 xenografts in immunocompromised mice was assessed when intraperitoneally administered. it was found that coti-2 at 10 mg/kg could significantly inhibit tumor growth in the ht-29 human colorectal tumor xenografts. in addition to reducing tumor volumes at specific times post-treatment, coti-2 could also delay the time required for tumors to reach specified volumes [1].
References
[1] salim, k. y.,vareki, s.m.,danter, w.r., et al. coti-2, a novel small molecule that is active against multiple human cancer cell lines in vitro and in vivo. oncotarget 7(27), (2016).
[2] https://clinicaltrials. gov/ct2/show/nct02433626term=coti-2&rank=1
