Detection of the egg yolk precursor Vtg in blood and tissue samples of juvenile and male fish is a simple and sensitive biomarker for endocrine disrupting chemicals (EDCs) with estrogenic effects in fish. Quantification of Vtg has become an accepted screening test for the estrogenic and anti-androgenic effects of EDCs in fish. The Vtg (fathead minnow) ELISA kit is a double-antibody immunometric (sandwich) ELISA for analyzing Vtg in whole body homogenates samples. Each kit contains fathead minnow Vtg standard, pre-coated plate(s), blocking/dilution buffer, detecting antibody-AChE conjugate, TMB substrate, PBS-Tween tablets, and complete instructions.WARNING This product is not for human or veterinary use.