Пируваткиназа

Химические свойства

White powder

Использование

Pyruvate kinase has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein expression. The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase. It is involved in glycolysis and in gluconeogenesis. It has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. It is also used to study pyruvate kinase (PK) deficiency.

Общее описание

Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M₁, and M₂. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M₁ are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M₂ is localized in the nucleus of the cells.

Методы очистки

These are purified by (NH4)2SO4 fractionation and gel filtration, ion-exchange and affinity chromatography. [Garcia-Olalla & Garrido-Pertierra Biochem J 241 573 1987.]