Флавин аденин динуклеотид динатриевая сол

Химические свойства

yellow-orange powder

Использование

The prosthetic group of certain flavoproteins including D-amino acid oxidase, glucose oxidase, glycine oxidase, fumaric hydrogenase, histaminase, and xanthine oxidase. Riboflavin kinase tumor necrosis factor receptor 1 NADPH oxidase.

прикладной

Flavin adenine dinucleotide disodium salt is the sodium salt form of Flavin adenine dinucleotide (FAD). FAD is used by flavoproteins as redox cofactors (electron carriers), including succinate dehydrogenase (complex), α-ketoglutarate dehydrogenase, apoptosis inducing factor 2 (AIF-M2, AMID), folate/FAD-dependent tRNA methyltransferase, and N-hydroxylated flavoprotein monooxygenase. FAD is a component of the pyruvate dehydrogenase complex. Flavin adenine dinucleotide disodium salt hydrate has been used as a component of plant tissue extraction buffers.

Методы очистки

Small quantities of FAD are purified by paper chromatography using tert-butyl alcohol/water, cutting out the main spot and eluting with water. Larger amounts can be precipitated from water as the uranyl complex by adding a slight excess of uranyl acetate to a solution at pH 6.0, dropwise and with gentle stirring. The solution is set aside overnight in the cold, and the precipitate is centrifuged off, washed with small portions of cold EtOH, then with cold peroxide-free diethyl ether. It is dried in the dark under vacuum over P2O5 at 50-60o. The uranyl complex is suspended in water, and, after adding sufficient 0.01M NaOH to adjust the pH to 7, the precipitate of uranyl hydroxide is removed by centrifugation [Huennekens & Felton Methods Enzymol 3 954 1957]. It can also be crystallised from water. It should be kept in the dark. More recently it was purified by elution from a DEAE-cellulose (Whatman DE 23) column with 0.1M phosphate buffer pH 7, and the purity was checked by TLC. [Holt & Cotton, J Am Chem Soc 109 1841 1987, Beilstein 26 III/IV 3632.]