ДИГИТОНИН

Химические свойства

white crystalline powder

Использование

Digitonin has been used in a study to assess a procedure for isolating mitochondria from ascites tumor cells. It has also been used in a study to investigate its effect on the cytotoxicity of plant secondary metabolites in cancer cells. This mild nonionic detergent can be used to solubilize receptors and permeabilize cellular and nuclear membranes.

Общее описание

Crystals or white powder.

Реакции воздуха и воды

Forms soapy aqueous suspension .

Профиль реактивности

DIGITONIN is incompatible with strong oxidizing agents and strong acids. (NTP, 1992

Пожароопасность

Flash point data for DIGITONIN are not available; however, DIGITONIN is probably combustible.

Профиль безопасности

Poison by ingestion, intravenous, intraperitoneal, and subcutaneous routes. Mutation data reported. See also DIGITALIS. When heated to decomposition it emits acrid smoke and irritating fumes.

Методы очистки

This digitoxin hexa-glycoside can be recrystallised from aqueous 85% EtOH or MeOH/diethyl ether. It is purified by preparative paper chromatography and developed with the upper phase of a mixture of nBuOH/H2O/AcOH (4:5:1), and the spot (RF 0.36) is eluted with 25% CCl3CO2H in CHCl3. It has also been purified by countercurrent distribution. It forms an ethanolate, and complexes with cholesterol and other sterols. [Ruhenstroth-Bauer & Breitenfeld Hoppe Seyler's Z Physiol Chem 302 111 1955, Grisvold J Am Pharm Assoc 23 664 1934, Beilstein 19 IV 1243.]