Агароза

Описание

Agarose is the neutral polymer component of agar. Agar belongs to the group of galactopolysaccharides, which are the intracellular matrix of red algae/seaweeds of the Rhodophyta (red algae phylum). Agar is naturally slightly sulphated and is a complex mixture of polysaccharides. Agarose forms a solution when heated and condenses into a gel when cooled. Gel formation is due to the formation of a double helix of molecular chains, which creates a water-fixing network. Agarose is commonly used for the analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern), and is also suitable for protein analyses such as Ouchterlony and Radial Immunodiffusion (RID). Ethidium bromide and SYBR Green background staining is low.

Химические свойства

Granular free flowing

Использование

Agarose is frequently used in molecular biology for the separation of large molecules, especially DNA, by electrophoresis. Slabs of agarose gels (usually 0.7 - 2%) for electrophoresis are readily prepared by pouring the warm, liquid solution into a mold. A wide range of different agaroses, of varying molecular weights and properties are commercially available for this purpose. Used in high resolution protein electrophoresis, immunoelectrophoresis, electro immunoassays such as rocket assays, counter current electrophoresis.

Определение

agarose: A carbohydrate polymerthat is a component of agar. It is usedin chromatography and electrophoresis.

Общее описание

Agarose, a natural polymer is obtained from sea algae. This neutral linear polysaccharide is made up of?d-galactose and 3,6-anhydro-l-galactose. These are connected alternatively with the help of glycosidic bonds. Agarose has high significance in gel electrophoresis and gel chromatography.

Сельскохозяйственное использование

Agarose is a partially methylated neutral polysaccharide used for making gel.