L-гистидин гидрохлорид моногидра

Химические свойства

white crystals or cryst. or granular powder

Использование

L-Histidine is used in cell culture media formulations used in biomanufacturing. L-Histidine has been used to study cultures of the human T-lymphoblastic leukemia cell line MOLT-4 to study modulation of apoptosis.

Общее описание

Chemicals play an important role in the stabilization of a biologic drug during its manufacturing and formulation process – for instance, by preventing aggregation. We offer a wide range of high-quality stabilizers, buffers and salts to successfully purify and formulate your biomolecules. Specifically developed for high-risk applications, they are low in bioburden and endotoxins.
As part of our Emprove^? Program, our raw materials are offered with extensive documentation facilitating compliance of your pharma and biopharma product, full supply chain transparency and risk mitigation. Our SAFC^? portfolio of high-quality products for biopharmaceutical and pharmaceutical formulation and production withstands strict quality control procedures and is produced according to applicable cGMP guidelines.

Методы очистки

Crystallise the monohydrochloride from aqueous EtOH or 60% aqueous EtOH (m 259odec). Alternatively dissolve 10g in 50mL of H2O, decolourise with Norite, filter, evaporate it in a vacuum to a syrup, cool to room temperature, add 95% EtOH with stirring until slightly turbid, scratch the sides of the vessel until crystals form, then add slowly 40mL of EtOH and keep at 0o overnight, filter the solid off, wash it several times with EtOH and dry it in a vacuum. [Cox J Biol Chem 78 475 1929, Cox et al. J Biol Chem 81 73 1929, Greenstein & Winitz The Chemistry of the Amino Acids J. Wiley, Vol 3 pp 1972, 2098 1961, Beilstein 25 II 407, 25 III/IV 4346.]