Description
1,N
6-Ethenoadenosine 5'-monophosphate (1,N
6-ε-AMP) is a highly fluorescent analog of adenosine 5’-monophosphate (AMP; ).
1,2 1,N
6-ε-AMP is characterized by a long fluorescent lifetime, detectability at low concentration, a relatively long wavelength of excitation (250-300 nm), and emission at 415 nm.
2,3,4 1,N
6-ε-AMP can be used in assays of 5’-nucleotidase activity.
5,6
Uses
1,N6-Ethenoadenosine 5'-monophosphate (1,N6-Etheno-AMP) sodium is a highly fluorescent analog of adenosine 5'-monophosphate (AMP). 1,N6-Ethenoadenosine 5'-monophosphate sodium is a powerful probe for systems involving adenosine 5'-monophosphate and can be detected at low concentration. 1,N6-Ethenoadenosine 5'-monophosphate sodium has long wavelength of excitation (250-300 nm), and emission at 415 nm[1][2].
References
1. Jahnz-Wechmann, Z., Framski, G.R., Januszczyk, P.A., et al.
Base-modified nucleosides: Etheno derivatives Front. Chem. 4,19(2016).
2. Secrist, J.A., III, Barrio, J.R., Leonard, N.J., et al.
Fluorescent modification of adenosine 3',5'-monophosphate: spectroscopic properties and activity in enzyme systems Science 177(4045),279-280(1972).
3. Penzer, G.R.
The solution conformation and some spectroscopic properties of 1,N 6 -ethenoadenosine monophosphate, a fluorescent analogue of AMP Eur. J. Biochem. 34(2),297-305(1973).
4. Spencer, R.D., Weber, G., Tolman, G.L., et al.
Species responsible for the fluorescence of 1:N6-ethenoadenosine Eur. J. Biochem. 45(2),425-429(1974).
5. Jamaz, Z., Afkham-Ebrahimi, A., and Saggerson, E.D.
A novel assay for 5'-nucleotidase using 1,N6-etheno-AMP as substrate, and comments on the properties of the reaction product, ethenoadenosine Biochem J. 250(2),369-373(1988).
6. Pexa, A., and Deussen, A.
Modulation of ecto-5'-nucleotidase by phospholipids in human umbilical vein endothelial cells (HUVEC) Naunyn-Schmiedeberg's Arch. Pharmacol. 372(2),131-138(2005).
