1-乙酰氧基-2,5-二氧代吡咯烷-3-磺酸

英文名称： Sulfo-NHS-Acetate

3-Sulfosuccinimid-1-yl acetate

1-Acetyloxy-2,5-dioxopyrrolidine-3-sulfonic acid

中文名称：1-乙酰氧基-2,5-二氧代吡咯烷-3-磺酸

CAS NO： 152305-87-8

MDL Number：MFCD01861959

分子式： C6H6NNaO7S

分子量： 259.2

纯 度： ≥95%

结构式:

性    状:白色或微黄色粉状制剂 

用    途：仅供科研实验使用，不用于诊治 

注意事项：取用一定要干燥，避免频繁的溶解和冻干 

溶解性：溶于大部分有机溶剂，如：DCM、DMF、DMSO、THF等等。在水中有很好的溶解性。

简  介：蛋白质交联剂是一类小分子化合物，具有2个或者更多的针对特殊基团（-NH2、-COOH、-HS等）的反应性末端，可以和2个或者更多的分子分别偶联，从而使这些分子结合在一起，终形成共轭蛋白。

1. Dissolve protein at a concentration of 1 – 10 mg/mL in 100 mM sodium phosphate buffer, pH 7.0- 8.0. Avoid amine containing buffers, such as Tris, glycine, or imidazole, etc. 

2. Create a 50 mg/mL Sulfo-NHS-Acetate stock solution in buffer (same buffer as in step one). 

3. To the protein solution, add 2 μL of the Sulfo-NHS-Acetate stock for every mg of protein dissolved. (ie. 10mg of protein = 5 μL Sulfo-NHS-Acetate stock). Note: Alternatively, you can add an equivalent amount of Sulfo-NHS-Acetate to the protein solution (mg of protein = mg of Sulfo-NHS-Acetate). 

4. Allow this reaction to proceed for 1-2 hours at room temperature. (2-3 hours if protein stability issues require 4°C incubation). 

5. If desired, residual Sulfo-NHS-Acetate can be deactivated by adding 0.5M Tris-HCl, pH 7.4 – 8.0 or 0.5M glycine to this reaction mixture. This is redundant if removing Sulfo-NHS-Acetate by desalting (see step 6). 

6. Desalt sample to remove residual Sulfo-NHS-Acetate and reaction bi-products (i.e. gel filtration, dialysis, etc.). Note: As a general rule for amine blocking with Sulfo-NHS-Acetate, 10-50 molar excess of SulfoNHS-Acetate to amines to be blocked will provide sufficient coverage.

产品列表：

Sulfo-NHS    CAS #: 106627-54-7
BS3               CAS #: 82436-77-9
BS2G
BSG              CAS #: 79642-50-5
DSP              CAS #: 57757-57-0
DSS              CAS #: 68528-80-3
DTSSP          CAS #: 81069-02-5
EGS              CAS #: 70539-42-3
Sulfo-EGS    
DSSeb           CAS # 23024-29-5
BMPS            CAS #: 55750-62-4
EMCS            CAS #: 55750-63-5
GMBS           CAS #: 80307-12-6
LC-SPDP             
MBS              CAS #: 58626-38-3
PDPH           
SBA               CAS #: 42014-51-7     
SIA                CAS #: 39028-27-8
SMCC            CAS #: 64987-85-5
SMPB            CAS #: 79886-55-8
SMPH           
SPDP             CAS #: 68181-17-9
Sulfo-LC-SPDP    
Sulfo-MBS    
Sulfo-SANPAH     CAS #: 102568-43-4
Sulfo-SMCC  CAS #: 92921-24-9
BS2G-d4
BS3-d4
DSG-d4
DSS-d4
DSP-d8
Biotin Hydrazide CAS #: 66640-86-6
NHS-Biotin       CAS #: 35013-72-0
NHS-SS-Biotin      CAS #: 142439-92-7
Sulfo-NHS-Biotin CAS #: 119616-92-5
Sulfo-NHS-LC-Biotin    CAS #: 128062-22-0
Sulfo-NHS-SS-Biotin    CAS #: 202057-28-1
HPG               CAS #: 24645-80-5
SATA              CAS #: 76931-93-6
Sulfo-NHS Acetate CAS #: 152305-87-8