BL21-Gold (DE3)pLysS 感受态细胞

BL21 and BL21-Gold-derived competent cells are designed for high-level protein expression using T7 RNA polymerase-based expression systems.‡‡ The BL21(DE3)pLysS and BL21-Gold(DE3)pLysS competent cells provide tighter control for expression of toxic proteins. When used with the CE6 bacteriophage, the BL21 and BL21-Gold cells provide the tightest control of protein expression, important for extremely toxic proteins. In addition, the BL21 and BL21-Gold cells can be used for non-T7 RNA polymerase protein expression systems. All BL21 strains are deficient in the OmpT and Lon proteases, which may interfere with isolation of intact recombinant proteins. Use the BL21 or the BL21-Gold competent cells and its derivatives with T7 promoter-driven vectors, such as the Affinity® pCAL vectors and pET vectors.