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  • pEGFP-N1 载体

pEGFP-N1 载体

pEGFP-N1
1000 2ug 起订
上海 更新日期:2024-03-29

上海泽叶生物科技有限公司

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产品详情:

中文名称:
pEGFP-N1 载体
英文名称:
pEGFP-N1
保存条件:
常温运输
纯度规格:
99%
产品类别:
质粒/载体

pEGFP-N1 载体

质粒类型:
荧光蛋白报告载体
启动子:
CMV
表达水平:
克隆方法:
多克隆位点,限制性内切酶
载体大小:
4733 bp (查看载体序列)
5' 测序引物及序列:
CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'
3' 测序引物及序列:
EGFP-N: 5'-CGTCGCCGTCCAGCTCGACCAG-3'
载体标签:
C-EGFP
载体抗性:
Kanamycin (卡那霉素)
筛选标记:
Neomycin (新霉素)
产品编号
产品名称
规格
价格
ZY1110
pEGFP-N1
2ug
 
pEGFP-N1 质粒图谱

pEGFP-N1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. The coding sequence of the EGFP gene contains more than 190 silent base changes which correspond to human codon-usage preferences (5). Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site (6) to further increase the translation efficiency in eukaryotic cells. The MCS in pEGFP-N1 is between the immediate early promoter of CMV (PCMV IE) and the EGFP coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. SV40 polyadenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the EGFP mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen. A neomycin-resistance cassette (Neor ), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene, allows stably transfected eukaryotic cells to be selected using G418. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. The pEGFP-N1 backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.

载体应用

Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo . The target gene should be cloned into pEGFP-N1 so that it is in frame with the EGFP coding sequences, with no intervening in-frame stop codons. The inserted gene should include the initiating ATG codon. The recombinant EGFP vector can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 (7). pEGFP-N1 can also be used simply to express EGFP in a cell line of interest (e.g., as a transfection marker).

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!

pEGFP-N1 载体/pEGFP-N1 质粒

公司简介

上海泽叶生物科技有限公司是一家服务于生命科学领域的高新技术企业,主要为科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学、材料科学,通用试剂、药物合成试剂、手性化合物、催化剂及配体、分析试剂、生物试剂,检测试剂等等

成立日期 (8年)
注册资本 100万元整
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,试剂,定制,服务
主营行业 生物化工,有机原料,化学试剂,医药原料,技术服务

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