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  • pEBFP-N1 载体

pEBFP-N1 载体

pEBFP-N1
1280 2ug 起订
上海 更新日期:2024-07-29

上海泽叶生物科技有限公司

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产品详情:

中文名称:
pEBFP-N1 载体
英文名称:
pEBFP-N1
保存条件:
常温运输
纯度规格:
99%
产品类别:
质粒/载体

pEBFP-N1 载体

质粒类型:
荧光蛋白报告载体
启动子:
CMV
克隆方法:
多克隆位点,限制性内切酶
载体大小:
4.7kb (查看载体序列)
启动子:
CMV
5' 测序引物及序列:
5'd[CGTCGCCGTCCAGCTCGACCAG]3'
载体抗性:
Kanamycin (卡那霉素)
筛选标记:
新霉素(Neomycin)
产品编号
产品名称
规格
价格
ZY1117
pEBFP-N1
2ug
 
pEBFP-N1 Vector

pEBFP-N1 carries a blue fluorescent variant of the Aequorea victoria green fluorescent protein gene (GFP). The EBFP gene contains four amino acid substitutions. The Tyr-66 to His substitution gives EBFP fluorescence excitation and emission maxima (380 and 440 nm, respectively) similar to other blue emission variants (1–3). The other three substitutions (Phe-64 to Leu; Ser-65 to Thr; and Tyr- 145 to Phe) enhance the brightness and solubility of the protein, primarily due to improved proteinfolding properties and efficiency of chromophore formation (1, 4, 5). The Em of EBFP is 31,000 cm–1M–1 for 380-nm excitation, leading to a fluorescent signal that is 2–3-fold brighter than other blue variants of GFP and roughly equivalent to wt GFP. In addition, the rate of photobleaching of EBFP is one-half to one-third that of P4-3, a popular predecessor to EBFP (1). EBFP contains >190 silent mutations that create an open reading frame comprised almost entirely of preferred human codons (6). Furthermore, upstream sequences flanking EBFP have been converted to a Kozak consensus translation initiation site (7). These changes increase the translational efficiency of the EBFP mRNA and consequently the expression of EBFP in mammalian and plant cells.

The MCS in pEBFP-N1 is between the immediate early promoter of CMV (PCMV IE) and the EBFP coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of EBFP if they are in the same reading frame as EBFP and there are no intervening stop codons. The inserted gene should include an initiating ATG codon. EBFP with N-terminal fusion moieties retain the fluorescent properties of the native protein and thus can be used to localize fusion proteins in vivo.

The vector contains an SV40 origin for replication and a neomycin resistance (Neor ) gene for selection (using G418) in mammalian cells. A bacterial promoter upstream of this cassette (P) expresses kanamycin resistance in E. coli. The vector backbone also provides a pUC19 origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!

pEBFP-N1 载体/pEBFP-N1质粒

公司简介

上海泽叶生物科技有限公司是一家服务于生命科学领域的高新技术企业,主要为科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学、材料科学,通用试剂、药物合成试剂、手性化合物、催化剂及配体、分析试剂、生物试剂,检测试剂等等

成立日期 (8年)
注册资本 100万元整
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,试剂,定制,服务
主营行业 生物化工,有机原料,化学试剂,医药原料,技术服务

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