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  • pTRE-Tight 载体

pTRE-Tight 载体

pTRE-Tight
1000 2ug 起订
上海 更新日期:2024-11-08

上海泽叶生物科技有限公司

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产品详情:

中文名称:
pTRE-Tight 载体
英文名称:
pTRE-Tight
保存条件:
常温运输
纯度规格:
99%
产品类别:
质粒/载体

pTRE-Tight 载体

质粒类型:
四环素调控系统
高拷贝/低拷贝:
低拷贝
克隆方法:
多克隆位点,限制性内切酶
载体大小:
2605 bp (查看载体序列)
载体抗性:
Ampicillin (氨苄青霉素)
产品编号
产品名称
规格
价格
ZY1605
pTRE-Tight
2ug
 
pTRE-Tight 质粒图谱

pTRE-Tight is a response plasmid that can be used to express a gene of interest (Gene X) in our BD™ Tet-On and BD™ Tet-Off Gene Expression Systems and Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossen et al. (3; Tet-On). pTRE-Tight contains an MCS immediately downstream of the Tet-responsive Ptight promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively. Ptight contains a modified Tet response element (TREmod), which consists of seven direct repeats of a 36-bp sequence that contains the 19-bp tet operator sequence (tetO). The TREmod is just upstream of the minimal CMV promoter (PminCMV), which lacks the enhancer that is part of the complete CMV promoter. Consequently, Ptight is silent in the absence of binding of TetR or rTetR to the tetOsequences. Note that the cloned insert must have an initiating ATG codon. In some cases, addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. pTRE-Tight-Gene X plasmids should be cotransfected with the Linear Hygromycin Marker (#631625 or #6202-1, not included) or Linear Puromycin Marker (#631626 or #6203-1, not included) to permit selection of stable transfectants. pTRE-Tight was derived from pTRE, originally described as pUHD10-3 in reference 5.

The pTRE-Tight-Luc Control Vector, packaged with the pTRE-Tight Vector, contains an additional 1,649 bp encoding firefly luciferase inserted into the MCS. This vector can be used as a reporter of induction efficiency using standard luciferase detection reagents. It is not intended as a cloning vector.

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!

pTRE-Tight 载体/pTRE-Tight质粒

公司简介

上海泽叶生物科技有限公司是一家服务于生命科学领域的高新技术企业,主要为科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学、材料科学,通用试剂、药物合成试剂、手性化合物、催化剂及配体、分析试剂、生物试剂,检测试剂等等

成立日期 (9年)
注册资本 100万元整
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,试剂,定制,服务
主营行业 生物化工,有机原料,化学试剂,医药原料,技术服务

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