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pHD_v2 载体

pHD_v2
1280 2ug 起订
上海 更新日期:2024-07-29

上海泽叶生物科技有限公司

VIP5年
联系人:宛双凤
电话:021-61998551拨打
手机:13122364865 拨打
邮箱:sale1@shzysw.net

产品详情:

中文名称:
pHD_v2 载体
英文名称:
pHD_v2
保存条件:
常温运输
纯度规格:
99%
产品类别:
质粒/载体

pHD_v2 载体

质粒类型:
Tale ToolBox kit
高拷贝/低拷贝:
高拷贝
克隆方法:
多克隆位点,限制性内切酶
载体大小:
2746 bp (查看载体序列)
载体抗性:
Kanamycin (卡那霉素)
产品编号
产品名称
规格
价格
ZY1619
pHD_v2
2ug
 
pHD_v2 质粒图谱

(a) Natural structure of TALEs derived from Xanthomonas sp. Each DNA-binding module consists of 34 amino acids, where the RVDs in the 12th and 13th amino acid positions of each repeat specify the DNA base being targeted according to the cipher NG = T, HD = C, NI = A, and NN = G or A. The DNA-binding modules are flanked by nonrepetitive N and C termini, which carry the translocation, nuclear localization (NLS) and transcription activation (AD) domains. A cryptic signal within the N terminus specifies a thymine as the first base of the target site.

(b) The TALE toolbox allows rapid and inexpensive construction of custom TALE-TFs and TALENs. The kit consists of 12 plasmids in total: four monomer plasmids to be used as templates for PCR amplification, four TALE-TF and four TALEN cloning backbones corresponding to four different bases targeted by the 0.5 repeat. CMV, cytomegalovirus promoter; N term, nonrepetitive N terminus from the Hax3 TALE; C term, nonrepetitive C terminus from the Hax3 TALE; BsaI, type IIs restriction sites used for the insertion of custom TALE DNA-binding domains; ccdB + CmR, negative selection cassette containing the ccdB negative selection gene and chloramphenicol resistance gene; NLS, nuclear localization signal; VP64, synthetic transcriptional activator derived from VP16 protein of herpes simplex virus; 2A, 2A self-cleavage linker; EGFP, enhanced green fluorescent protein; polyA signal, polyadenylation signal; FokI, catalytic domain from the FokI endonuclease.

(c) TALEs can be used to generate custom TALE-TFs and modulate the transcription of endogenous genes from the genome. The TALE DNA-binding domain is fused to the synthetic VP64 transcriptional activator, which recruits RNA polymerase and other factors needed to initiate transcription.

(d) TALENs can be used to generate site-specific double-strand breaks to facilitate genome editing through nonhomologous repair or homology directed repair. Two TALENs target a pair of binding sites flanking a 16-bp spacer. The left and right TALENs recognize the top and bottom strands of the target sites, respectively. Each TALE DNA-binding domain is fused to the catalytic domain of FokI endonuclease; when FokI dimerizes, it cuts the DNA in the region between the left and right TALEN-binding sites.

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!

pHD_v2 载体/pHD_v2质粒

公司简介

上海泽叶生物科技有限公司是一家服务于生命科学领域的高新技术企业,主要为科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学、材料科学,通用试剂、药物合成试剂、手性化合物、催化剂及配体、分析试剂、生物试剂,检测试剂等等

成立日期 (8年)
注册资本 100万元整
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,试剂,定制,服务
主营行业 生物化工,有机原料,化学试剂,医药原料,技术服务

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