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  • pSV-β-Galactosidase 载体

pSV-β-Galactosidase 载体

pSV-β-Galactosidase
990 2ug 起订
上海 更新日期:2024-11-08

上海泽叶生物科技有限公司

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产品详情:

中文名称:
pSV-β-Galactosidase 载体
英文名称:
pSV-β-Galactosidase
保存条件:
常温运输
纯度规格:
99%
产品类别:
质粒/载体

pSV-β-Galactosidase 载体

别名:
pSV-Beta-Galactosidase
质粒类型:
哺乳细胞表达载体
克隆方法:
多克隆位点,限制性内切酶
载体大小:
6820 bp (查看载体序列)
载体抗性:
Ampicillin (氨苄青霉素)
产品编号
产品名称
规格
价格
ZY1967
pSV-β-Galactosidase
2ug
 
pSV-Beta-Galactosidase 质粒图谱

The pSV-β-Galactosidase Control Vector(a) is designed as a positive control vector for monitoring transfection efficiencies of mammalian cells. The SV40 early promoter and enhancer drive transcription of the bacterial lacZ gene, which in turn, is translated into the β-galactosidase enzyme. β-galactosidase is an excellent reporter enzyme (1,2), that can be assayed quickly and directly in cell extracts using spectrophotometric, fluorescent or chemiluminescent assays (3,4). This reporter enzyme is also widely used for in situ histochemical analysis using the substrate X-Gal (5).

The pSV-β-Galactosidase Control Vector can be co-transfected with your DNA of interest. For example, co-transfection with firefly luciferase gene vectors (pGL3 Vectors) provide cell extracts that can be assayed for both luciferase and β-galactosidase activities. In this manner, the pSV-β-Galactosidase Vector acts as an internal control for transient expression assays. A negative control extract, prepared from mock-transfected cells, should also be assayed for the presence of endogenous β-galactosidase activity in cultured cells (2). In addition, co-transfection with chloramphenicol acetyltransferse reporter gene vectors (pCAT®3 Vectors) permits assaying for both CAT and β-galactosidase activities.

The pSV-β-Galactosidase Vector is a modification of pRSV-βGAL (6) with SV40 and pUC18 sequences substituted for RSV and pBR322 sequences. The pSV-β- Galactosidase Vector will express β-galactosidase in E. coli due to the presence of the E. coli gpt promoter located upstream of the lacZ gene (1). Colonies of E. coli containing the pSV-β-Galactosidase Vector will appear blue when plated on media containing X-gal.

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pSV-β-Galactosidase 载体/pSV-β-Galactosidase质粒

公司简介

上海泽叶生物科技有限公司是一家服务于生命科学领域的高新技术企业,主要为科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学、材料科学,通用试剂、药物合成试剂、手性化合物、催化剂及配体、分析试剂、生物试剂,检测试剂等等

成立日期 (9年)
注册资本 100万元整
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,试剂,定制,服务
主营行业 生物化工,有机原料,化学试剂,医药原料,技术服务

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