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  • pDisplay 载体

pDisplay 载体

pDisplay
990 2ug 起订
上海 更新日期:2024-09-11

上海泽叶生物科技有限公司

VIP5年
联系人:宛双凤
电话:021-61998551拨打
手机:13122364865 拨打
邮箱:sale1@shzysw.net

产品详情:

中文名称:
pDisplay 载体
英文名称:
pDisplay
保存条件:
常温运输
纯度规格:
99%
产品类别:
质粒/载体

pDisplay 载体

质粒类型:
哺乳动物表面展示系统
启动子:
CMV
载体大小:
5325 bp (查看载体序列)
5' 测序引物及序列:
T7-F:TAATACGACTCACTATAGGG
载体标签:
HA Tag, c-Myc Epitope Tag, PDGFR Transmembrane Domain, IgK Leader Sequence
载体抗性:
Ampicillin (氨苄青霉素)
筛选标记:
Neomycin (新霉素)
产品编号
产品名称
规格
价格
ZY1628
pDisplay
2ug
 
pDisplay 质粒图谱

pDisplay™ is a mammalian expression vector designed to target recombinant proteins to the surface of mammalian cells. Displayed proteins can be analyzed for their ability to interact with known or putative ligands. Proteins of interest are targeted and anchored to the cell surface by cloning the gene of interest in frame with the vectors unique N-terminal secretion signal and the C-terminal transmembrane anchoring domain of platelet-derived growth factor receptor (PDGFR). In contrast to phage display vectors which operate exclusively in prokaryotic cells, the pDisplay™ vector offers the advantage of having the displayed protein of interest processed in mammalian cells. Therefore, recombinant proteins of eukaryotic origin that are expressed from pDisplay™ more closely resemble their native form. This may favor more accurate ligand binding interactions. In addition to the N-terminal cell surface targeting signal and C-terminal transmembrane anchoring domain, the pDisplay™ vector contains the following key features:

• T7 promoter/priming site for in vitro transcription of sense RNA and for sequencing of inserts
• Neomycin resistance marker for stable selection in mammalian cells using Geneticin®
• SV40 origin for replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1 and COS-7)
• Ampicillin resistance gene for selection in E. coli

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!

pDisplay

公司简介

上海泽叶生物科技有限公司是一家服务于生命科学领域的高新技术企业,主要为科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学、材料科学,通用试剂、药物合成试剂、手性化合物、催化剂及配体、分析试剂、生物试剂,检测试剂等等

成立日期 (9年)
注册资本 100万元整
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,试剂,定制,服务
主营行业 生物化工,有机原料,化学试剂,医药原料,技术服务

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