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  • pBI Tet 载体

pBI Tet 载体

pBI Tet
990 2ug 起订
上海 更新日期:2024-12-25

上海泽叶生物科技有限公司

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产品详情:

中文名称:
pBI Tet 载体
英文名称:
pBI Tet
保存条件:
常温运输
纯度规格:
99%
产品类别:
质粒/载体

pBI Tet 载体

质粒类型:
四环素调控系统
载体大小:
4.4kb
载体抗性:
Ampicillin (氨苄青霉素)
产品编号
产品名称
规格
价格
ZY1601
pBI Tet
2ug
 
pBI Tet质粒图谱

The pBI Tet Vector is a response plasmid that can be used to express two genes of interest from one bidirectional tet-responsive promoter (Pbi-1; 1) in Clontech’s Tet-On® and Tet-Off® Gene Expression Systems and Cell Lines (2). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (3; Tet-Off) and Gossen et al. (4; Tet-On). The pBI Tet Vector contains the bidirectional promoter Pbi-1 which is responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively. Pbi-1 contains the Tet-responsive element (TRE), which consists of seven copies of the 42-bp tet operator sequence (tetO). The TRE element is between two minimal CMV promoters (PminCMV), which lack the enhancer that is part of the complete CMV promoter. Consequently, Pbi-1 is silent in the absence of binding of TetR or rTetR to the tetO sequences. PminCMV-1 and PminCMV-2 control the expression of two separate genes of interest. Note that the cloned inserts must have an initiation codon. In some cases, addition of a Kozak consensus ribosome binding site (5) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence.

载体应用

pBI allows the simultaneous regulation of two genes of interest by one central TRE. After a stable Tet-On or Tet-Off cell line has been established by transfecting with a tTA or rtTA regulator plasmid, pBI is cotransfected with pTK-Hyg (Cat No. 631750) to permit selection of a double-stable cell line which expresses both genes of interest. Alternatively, pPUR (Cat No. 631601) or another selection plasmid can be used. If this plasmid contains an enhancer element, as does pPUR, cointegration of pBI and the selection plasmid may lead to higher background expression. Double-stable, tet-responsive cell lines with pBI response constructs can be developed using the protocols described for pTRE response plasmids in the Tet Systems User Manual (PT3001-1).

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!

pBI Tet 载体/pBI Tet质粒

公司简介

上海泽叶生物科技有限公司是一家服务于生命科学领域的高新技术企业,主要为科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学、材料科学,通用试剂、药物合成试剂、手性化合物、催化剂及配体、分析试剂、生物试剂,检测试剂等等

成立日期 (9年)
注册资本 100万元整
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,试剂,定制,服务
主营行业 生物化工,有机原料,化学试剂,医药原料,技术服务

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