多肽定制合成
Apeptide公司提供优质的多肽合成定制服务,可以根据客户要求合成不同纯度、不同长度的多肽。公司在多肽方面的技术国内领先,尤其在糖肽(Glycopeptide)、环肽(Cyclicpeptide)、甲基化多肽(Methyl-peptide)、磺化多肽(Sulfication-peptide)、磷酸化多肽(Phosphopeptides)、天然产物肽(Natural peptides)、各种荧光标记肽(Fluorescent Labeled Peptides)和修饰肽(modification Peptides)等方面有着领先的技术。
多肽定制服务 - small scale (typically < 1 gram) synthesis of peptides containing the standard 20 amino acids
多肽修饰服务 - small scale (typically < 1 gram) synthesis of modified peptides (e.g. N-acetylated, C-amidated, biotinylated, etc.) or peptides containing unusual or unnatural amino acids (e.g., phosphotyrosine, D-amino acids, beta-amino acids, etc...).
库肽 - able to produce peptide libraries or peptide arrays for screening purposes
MAPs - able to produce multiple antigenic peptides (MAPs)
多肽修饰
Apeptide 公司提供各种修饰,其主要的修饰方法和技术如下:
C-端标记技术
C端酰胺化(Amidation,C-termainal)
醛基化(Aldehydes)
醇基化(Alcohols)
pNA (p-Nitroanilide)
AMC
AFC
巯基乙胺化(Cysteamide)
酯基化(Ester)
N-烷基化(N-Alkyl Amides)
N-端标记技术
乙酰化Acetylated (Acetylated)
Palmytolyl
HYNIC
生物素标记(Biotinylated)
Br乙酰化(Bromoacetylated)
螯合反应(DOTA,DTPA conjugated)
甲醛化(Formylated)
十四烷基,十八烷基化(Myristoylated)
琥珀酰化、棕榈酸化、苹果酸化、脂肪酸化等(Succinylated)
荧光标记修饰
C端修饰
AFC, AMC, Dap(Dnp), Lys(Dye), pNA, Rh110
N端修饰
Bodipy-FL, Cy3, Cy5, Texas Red, 5-Tamra, 5-lodoacetamido fluorescein
Rhodamine 110 and Rhodamine B Luciferin, EDANS
FAM, FITC, MCA, Rox, Sulforhodamine 101, 5-TAMRA
环化反应
首尾成环,中间成环,特殊成环(N -> C or Head to Tail)
2对二硫键,3对二硫键(Disulfide (S-S bond formation) .Trisulfide formation)
天然产物活性肽成环(Cyclic-natural peptides
甲基化修饰
侧链甲基化Lys(For),Lys(Me), Lys(Me)2, Lys(Me)3, Arg(Me)2 symmetrical, D-Tyr(Me),D-Tyr(Et)
N端甲基化(N-Me-Arg,N-Me-Asp, N-Me-Glu, N-Me-Leu, N-Me-Nle, N-Me-Nva, N-Me-Phe, N-Me-S N- Me-Ser, N-Me-Trp, N-Me-Thr, N-Me-Val)
几种常见的特殊修饰
各种偶联(BSA, KLH conjugated peptides for antibody production )
磷酸化(Phosphoserine, Phosphothreonine, Phosphotyrosine)
磺化(Sulfated Tyrosine or Serine)
MAPS (Multiple Antigenic Peptide)
糖肽(Glycopeptides)
PEG修饰(PEGylation)