Recombinant Murine Bcl-xL

Name

Recombinant Murine Bcl-xL (Recombinant Murine B-cell Lymphoma-extra Large; rMuc)；Recombinant Mouse Bcl-xL ; 重组小鼠B细胞淋巴瘤xL

Synonyms

Apoptosis regulator Bcl-X; bcl2-L-1; Bcl2-L-1; BCL2-like 1; bcl-2-like protein 1; BCLXBCL2LBcl-X; BCLXL; bcl-xL; BCL-XL/S; BCLXS; bcl-xS; DKFZp781P2092

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Test in Process.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, 5% Trehalose.

Endotoxin

Less than 0.1EU/μg of rMuBcl-xL as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Bcl-X, also named as BCL2L1 or BCL2L, belongs to the Bcl-2 family and is encoded by the BCL2L1 gene in human. Alternative splicing of Bcl-X results in at least two isoforms，isoform Bcl-X(L) (also named as Bcl-xL) and isoform Bcl-x(S) (also named as Bcl-xS). Bcl-xL is found in tissues containing long-lived postmitotic cells, such as adult brain, while Bcl-xS is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. Bcl-X forms homodimer or heterodimer with other Bcl-2 proteins, like BAK, BAX or Bcl-2, to act as anti- or pro- apoptotic regulators. Bcl-xL appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane, and it also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. In contrast, Bcl-xS is a pro-apoptotic protein that promotes apoptosis.

Amino Acid Sequence

SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEET EAERETPSAI NGNPSWHLAD SPAVNGATGH SSSLDAREVI PMAAVKQALR EAGDEFELRY RRAFSDLTSQ LHITPGTAYQ SFEQVVNELF RDGVNWGRIV AFFSFGGALC VESVDKEMQV LVSRIASWMA TYLNDHLEPW IQENGGWDTF VDLYGNNAAA ESRKGQERFN 
R

产品编号

产品名称

包装

KL7343-2μg

Recombinant Murine Bcl-xL

2μg

KL7343-10μg

Recombinant Murine Bcl-xL

10μg

KL7343-100μg

Recombinant Murine Bcl-xL

100μg

KL7343-1mg

Recombinant Murine Bcl-xL

1mg

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说明书

1份