Recombinant Murine CT-1

Name

Recombinant Murine CT-1 (Recombinant Murine Cardiotrophin-1; rMuCT-1)；Recombinant Mouse CT-1 ; 重组小鼠心肌营养素1

Synonyms

CTF1; cardiophin 1; cardiotrophin 1; cardiotrophin-1; CT-1CT1

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5ng/ml, corresponding to a specific activity of >2.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris, 300mM NaCl, pH8.5.

Endotoxin

Less than 1EU/μg of rMuCT-1 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. In addition, CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Murine CT-1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide. Human and murine CT-1 share 80%a.a. sequence identity and exhibit crossspecies activity.

Amino Acid Sequence

MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC GLYGEWVSRT EGDLGQLVPG GVA

产品编号

产品名称

包装

KL7334-2μg

Recombinant Murine CT-1

2μg

KL7334-10μg

Recombinant Murine CT-1

10μg

KL7334-100μg

Recombinant Murine CT-1

100μg

KL7334-1mg

Recombinant Murine CT-1

1mg

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说明书

1份