Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is typically 1-5µg/cm2. Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50µg/ml. Optimal concentrations will need to be determined for individual user applications.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µM filtered concentrated solution in 20mM Tris-HCl, pH8.0, 150mM NaCl, with 5% Trehalose, 0.02% Tween-20.
Endotoxin
Less than 0.1EU/µg of PrimeNectin as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
A recombinant, single non-glycosylated polypeptide chain containing 574 amino acids from human fibronectin called PrimeNectin. Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis.