Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4, with 1mM DTT.
Endotoxin
Less than 0.1EU/μg of rHuGalectin-1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
Human Galectin-1 also named BHL, Galaptin, GBP, L-14 and LGALS1, is belonging to the galectins family and it is encoded by the LGALS1 gene in human. Galectin-1 is expressed by the endometrial stromal cells throughout the menstrual cycle. Galectin-1 contains a single carbohydrate recognition domain through which it can bind glycans both as a monomer and as a homodimer. Dimers are non-covenantly bound and will spontaneously disassociate in low concentration. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 is thought to play a role in the immunosuppression required for a successful pregnancy. Human Galectin-1 shares 88%-90% amino acid sequence identity with rat and mouse.