Recombinant β-lactamase TEM-1

Name

Recombinant β-lactamase TEM-1 (Recombinant β-lactamase TEM-1; rTEM-1)；重组β-内酰胺酶TEM-1

Synonyms

Beta-lactamase TEM; IRT-4; Penicillinase; TEM-1; TEM-16/CAZ-7; TEM-2; TEM-24/CAZ-6; TEM-3; TEM-4; TEM-5; TEM-6; TEM-8/CAZ-2; bla; blaT-3; blaT-4; blaT-5; blaT-6

Purity

>95% by SDS-PAGE.

Biological Activity

Fully biologically active when compared to standard. One unit of enzyme activity is defined as the amount of enzyme which will hydrolyze 1.0μmol of benzyl penicillin in presence of EDTA at pH 7.0 and at 25℃.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 100mM Tris, pH7.0.

Endotoxin

N/A

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Enzymes

Background

Beta-lactamases are enzymes produced by some bacteria and are responsible for their resistance to beta-lactam antibiotics like penicillins, cephamycins, and carbapenems. The lactamase enzyme breaks the β-lactam ring open and deactivates the molecule's antibacterial properties because of a common element in these antibiotics molecular structure: a four-atom ring known as a betalactam. TEM-1 is the most commonly-encountered beta-lactamase in gram-negative bacteria. Up to 90% of ampicillin resistance in E. coli is due to the production of TEM-1. Also responsible for the ampicillin and penicillin resistance that is seen in H.influenzae and N.gonorrhoeae in increasing numbers. Based upon different combinations of changes, currently 140 TEM-type enzymes have been described. Recombinant beta-lactamase TEM-1 contains 264 amino acids residues.

Amino Acid Sequence

MHPETLVKVK DAEDQLGARV GYIELDLNSG KILESFRPEE RFPMMSTFKV LLCGAVLSRV DAGQEQLGRR IHYSQNDLVE YSPVTEKHLT DGMTVRELCS AAITMSDNTA ANLLLTTIGG PKELTAFLHN MGDHVTRLDR WEPELNEAIP NDERDTTMPA AMATTLRKLL TGELLTLASR QQLIDWMEAD KVAGPLLRSA LPAGWFIADK SGAGERGSRG IIAALGPDGK PSRIVVIYTT GSQATMDERN RQIAEIGASL IKHW

产品编号

产品名称

包装

KL7082-1mg

Recombinant β-lactamase TEM-1

1mg

KL7082-5mg

Recombinant β-lactamase TEM-1

5mg

KL7082-10mg

Recombinant β-lactamase TEM-1

10mg

KL7082-100mg

Recombinant β-lactamase TEM-1

100mg

-

说明书

1份