Recombinant Human UBE2I, His-tag

Name

Recombinant Human UBE2I, His-tag (Recombinant Human SUMO-Conjugating Enzyme UBC9, His-tag; rHuUBE2I, His-tag)；重组人泛素结合酶E2I，His标签

Synonyms

p18; SUMO-protein Ligase; Ubc9; UBCE9; C358B7.1; EC 6.3.2; EC 6.3.2.-; P18; p18; SUMO-1-protein ligase; SUMO-protein ligase; UBC9SUMO-conjugating enzyme UBC9; UBCE9; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; Ubiquitin-protein ligase I

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Data is not available.

Physical Appearance

Sterile Colorless liquid.

Formulation

A 0.2μm filtered concentrated solution in 50mM HEPES, pH7.6, with 125mM NaCl, 10% Glycerol, 1mM DTT.

Endotoxin

Less than 1EU/μg of rHuUBC9, His-tag as determined by LAL method.

Reconstitution

N/A

Category

Ubiquitins

Background

SUMO-Conjugating Enzyme UBC9 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2I gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBC9 accepts the ubiquitin-like proteins SUMO1-4 from the UBLE1AUBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Additionally, It takes parts in catalysis the formation of poly-SUMO chains, sumoylation of FOXL2 and KAT5, and the segregation of nuclear architecture and chromosome.

Amino Acid Sequence

MHHHHHHAMG TLNMSGIALS RLAQERKAWR KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP S

产品编号

产品名称

包装

KL7113-10μg

Recombinant Human UBE2I, His-tag

10μg

KL-7113-50μg

Recombinant Human UBE2I, His-tag

50μg

KL-7113-100μg

Recombinant Human UBE2I, His-tag

100μg

KL-7113-1mg

Recombinant Human UBE2I, His-tag

1mg

-

说明书

1份