Recombinant Human MIC-A, His-tag

Name

Recombinant Human MIC-A, His-tag (Recombinant Human MHC Class I PolypeptideRelated Sequence A, His-tag; rHuMIC-A, His-tag)；重组人MIC类多肽相关序列A，His标签

Synonyms

FLJ60820; MGC111087; PERB11.1

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The specific activity is determined by binding MICA antibody in ELISA.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, and 8 M Urea.

Endotoxin

Less than 1EU/μg of rHuMIC-A, His-tag as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions, which contain 8M Urea.

Category

Others

Background

MIC-A (MHC class I chain-related gene A) is a single-pass type I member protein. It is expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Additionally, MIC-A can be induced by bacterial and viral infections. It shares 85% amino acid identity with MIC-B and they are distantly related to the MHC class I proteins. Because they possess three extracellular Ig-like domains, but unlike classical MHC class I molecules. They do not form a heterodimer with beta2 microglobulin, but bind as a monomer to a KLRK1/NKG2D that is an activating receptor expressed on NK cells, NKT cells, γδ T cells, and CD8⁺αβ T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MIC-A recognition plays an important role in tumor surveillance, viral infections, and autoimmune diseases.

Amino Acid Sequence

MSYYHHHHHH DYDIPTTENL YFQGAMAPEF EPHSLRYNLT VLSWDGSVQS GFLTEVHLDG QPFLRCDRQK CRAKPQGQWA EDVLGNKTWD RETRDLTGNG KDLRMTLAHI KDQKEGLHSL QEIRVCEIHE DNSTRSSQHF YYDGELFLSQ NLETKEWTMP QSSRAQTLAM NVRNFLKEDA MKTKTHYHAM HADCLQELRR YLKSGVVLRR TVPPMVNVTR SEASEGNITV TCRASGFYPW NITLSWRQDG VSLSHDTQQW GDVLPDGNGT YQTWVATRIC QGEEQRFTCY MEHSGNHSTH PVPSGKVLVL QSH KLGCFGG

产品编号

产品名称

包装

KL-7145-10μg

Recombinant Human MIC-A, His-tag

10μg

KL-7145-50μg

Recombinant Human MIC-A, His-tag

50μg

KL-7145-100μg

Recombinant Human MIC-A, His-tag

100μg

KL-7145-1mg

Recombinant Human MIC-A, His-tag

1mg

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说明书

1份