Recombinant Human HMGB1, His-tag

Name

Recombinant Human HMGB1, His-tag (Recombinant Human High-Mobility Group Box 1, His-tag; rHuHMGB1, His-tag)；重组人高移动性型盒2，His标签

Synonyms

HMG-1; Amphoterin; high mobility group box 1; High mobility group protein 1; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG-1; HMG1DKFZp686A04236; HMG3; SBP-1; Sulfoglucuronyl carbohydrate binding protein

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Data not available.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuHMGB1, His-tag as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30kDa, 215 amino acid (a.a.) single chain polypeptide containing three domains: two N-terminal globular, 70a.a. positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30a.a. C-terminal region that contains only Asp and Glu.4, 5 Residues 27-43 and 178-184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.

Amino Acid Sequence

MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH

产品编号

产品名称

包装

KL7163-10μg

Recombinant Human HMGB1, His-tag

10μg

KL-7163-50μg

Recombinant Human HMGB1, His-tag

50μg

KL-7163-100μg

Recombinant Human HMGB1, His-tag

100μg

KL-7163-1mg

Recombinant Human HMGB1, His-tag

1mg

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说明书

1份