Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH8.0, 5% Trehalose.
Endotoxin
Less than 0.1EU/μg of rHuBcl-xL as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
Bcl-X, also named as BCL2L1 or BCL2L, belongs to the Bcl-2 family and it is encoded by the BCL2L1 gene in human. Alternative splicing of Bcl-X results in at least two isoforms，isoform Bcl-X(L) (also named as Bcl-xL) and isoform Bcl-x(S) (also named as Bcl-xS). Bcl-xL is found in tissues containing long-lived postmitotic cells, such as adult brain, while Bcl-xS is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. Bcl-X forms homodimer or heterodimer with other Bcl-2 proteins, like BAK, BAX or Bcl-2, to act as anti- or pro- apoptotic regulators. Bcl-xL appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane, and it also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. In contrast, Bcl-xS is a pro-apoptotic protein that promotes apoptosis.
