Recombinant Human IHH C28II (Recombinant Human Indian Hedgehog Cys28IleIle; rHuIHH C28II);重组人印度刺猬因子C28II
Synonyms
BDA1; HHG2; Ihh; BDA1Indian hedgehog homolog (Drosophila); HHG2; HHG-2; Indian hedgehog; Indian hedgehog (Drosophila) homolog; Indian hedgehog homolog; indian hedgehog protein
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10μg/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 1X PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuIHH C28II as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
IHH, encoded by the IHH gene in humans, belongs to the mammalian hedgehog family. It is expressed in adult kidney and liver. The function of IHH is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). IHH is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells. Human IHH shares 100% amino acid sequence identity with murine.