Recombinant Human UBE2M (Recombinant Human Ubiquitin-conjugating Enzyme E2 M; rHuUBE2M);重组人泛素结合酶E2M
Synonyms
UBC12; UbcH12; UBC-RS2; EC 6.3.2; EC 6.3.2.-; hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; UBC12NEDD8-conjugating enzyme Ubc12; UBC-RS2; ubiquitin carrier protein M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12); ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Data is not available.
Physical Appearance
Sterile Colorless liquid
Formulation
A 0.2μm filtered concentrated solution in 50mM HEPES, pH6.5, with 125mM NaCl, 10% Glycerol, 5% Trehalose, 1mM DTT.
Endotoxin
Less than 0.1EU/μg of rHuUBE2M as determined by LAL method.
Reconstitution
N/A
Category
Ubiquitins
Background
NEDD8-Conjugating Enzyme Ubc12 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2M gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBC12 accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Additionally, it involved in cell proliferation.