Recombinant Human TFF1 (Recombinant Human Trefoil Factor 1; rHuTFF1);重组人三叶因子1
Synonyms
BCEI; D21S21; HPS2; PNR-2; PS2; BCEIbreast cancer, estrogen-inducible sequence expressed in; Breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; hP1.A; HPS2; pNR-2; PNR-2; Polypeptide P1.A; Protein pS2; pS2; PS2; trefoil factor 1; trefoil factor, BCE1, human pS2 induced by estrogen from human breast cancercell line M10HP1.A
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10μg/ml, corresponding to a specific activity of >100IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 150mM NaCl.
Endotoxin
Less than 1EU/μg of rHuTFF1 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
Trefoil factor 1 belongs to the trefoil factor family that consists of three members named TFF1, TFF2 and TFF3. They are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. The TFFs are stable secretory proteins expressed highly in the gastrointestinal tract (gastric mucosa). TFF1 is an essential protein for normal differentiation of the antral and pyloric gastric mucosa and functions as a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. It is studied because of commonly expressed in tumors as well.