Recombinant Human PSA/Kallikrein-3 (Recombinant Human Prostate-Specific Antigen/Kallikrein-3; rHuPSA/Kallikrein-3);重组人前列腺特异性抗原/激肽释放酶-3
Synonyms
APS; PSA; hK3; KLK2A1; KLK3
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Data not available.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µM filtered concentrated solution in 20mM Tris-HCl, pH8.0, 150mM NaCl, 3% trehalose.
Endotoxin
Less than 0.1EU/µg of rHuPSA/Kallikrein-3 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Enzymes
Background
The human tissue kallikrein (KLK) gene family contains 15 members that play important roles in cancer. Kallikrein-3, called prostate specific antigen (PSA), is a glycoprotein enzyme encoded in humans by the KLK3 gene. PSA is secreted by the epithelial cells of the prostate gland, hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. PSA is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer.