Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CCR10 transfected BaF3 cells is in a concentration range of 10-100ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rHuCTACK/CCL27 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Chemokines
Background
CCL27, also named IL-11 R-alpha-locus chemokine (ILC), skinkine, eskine and cutaneous T-cell-attracting chemokine (CTACK), is a small cytokine belonging to the CC chemokine family. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin and it elicits its chemotactic effects by binding to the chemokine receptor CCR10. CCL27 can attract skin-associated memory T-lymphocytes. Studies showed it may play a role in mediating homing of lymphocytes to cutaneous sites and cell migration during embryogenesis. Mature human CCL27 is an 88 amino acid (a.a.) protein that shares 56%a.a. sequence identity with murine.