Recombinant Human TAFA-2 (Recombinant Human Chemokine-like protein TAFA-2; rHuTAFA-2);重组人趋化因子样蛋白TAFA2
Synonyms
FAM19A2; Chemokine-like protein TAFA-2; DKFZp761E1217; DKFZp781P0552; family with sequence similarity 19 (chemokine (C-C motif)-like), member A2; MGC42403; TAFA2; TAFA-2
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biological activity is determined by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. rHuTAFA-2, immobilized at 6-24μg/ml on a 96 well plate, is able to significantly enhance neurite outgrowth.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rHuTAFA-2 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Chemokines
Background
TAFA-2 also named FAM19A2, is a chemokine-like protein which is belonged to the FAM19/TAFA family. The family is a newly discovered and distantly related to MIP-1α. It contains 5 members and TAFA proteins are highly expressed in specific brain regions. Like other members of the TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues. Human TAFA2 is 97%a.a. identical to mouse TAFA2. The biological functions of TAFA family members remain to be determined, but they are postulated to act as brain-specific chemokines or neurokines that take parts in regulators of immune and nervous cells.