Recombinant Human RANTES/CCL5 (Recombinant Human Regulation upon Activation Normal T cell Express Sequence/CCL5; rHuRANTES/CCL5)；重组人正常T细胞表达和分泌因子/CCL5
Synonyms
RANTES; SISd; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 100mM NaCl.
Endotoxin
Less than 1EU/μg of rHuRANTES/CCL5 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Chemokines
Background
Chemokine (C-C motif) ligand 5 (CCL5) also known as RANTES is classified as a chemotactic cytokine or chemokine. It is encoded by the CCL5 gene in humans. CCL5 is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes into inflammatory sites which has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells. It also induces the proliferation and activation of certain natural-killer cells to form CHAK cells. Recombinant human CCL5 contains 68 amino acids and it shares 84%a.a. sequence identity with murine and rat CCL5, respectively.
