Recombinant Human MCP-1/CCL2

Name

Recombinant Human MCP-1/CCL2 (Recombinant Human Monocyte Chemotactic Protein-1/CCL2; rHuMCP-1/CCL2)；重组人单核细胞趋化蛋白1/CCL2

Synonyms

MCAF; MCP-1

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 100mM NaCl.

Endotoxin

Less than 1EU/μg of rHuMCP-1/CCL2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Chemokines

Background

CCL2, also named monocyte chemotactic protein-1 (MCP-1) and small inducible cytokine A2, is a small cytokine that belongs to the CC chemokine family. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa and it is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. Research shows that CCR2 and CCR4 are two cell surface receptors that bind CCL2. CCL2 exhibits a chemotactic activity for monocytes and basophils. However, it does not attract neutrophils or eosinophils. CCL2 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis and it may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Mature human CCL2 shares 78-79%a.a. identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49a.a. and shares only ~56%a.a. identity within the common region.

Amino Acid Sequence

QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

产品编号

产品名称

包装

KL-6573-5μg

Recombinant Human MCP-1/CCL2

5μg

KL-6573-20μg

Recombinant Human MCP-1/CCL2

20μg

KL-6573-100μg

Recombinant Human MCP-1/CCL2

100μg

KL-6573-1mg

Recombinant Human MCP-1/CCL2

1mg

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说明书

1份