Recombinant Rat VEGF120, Yeast (Recombinant Rat Vascular Endothelial Growth Factor 120, Yeast; rRtVEGF120, Yeast);重组大鼠血管内皮生长因子120,酵母
Synonyms
Vpf; Vegf; VEGFA
Purity
>95% by SDS-PAGE and 90% by SEC-HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 2‑10ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µM filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/µg of rRtVEGF120, Yeast as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Theoretically as a disulfide-linked homodimeric protein, the product consists of two 121 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 18.5kDa in SDS-PAGE under reducing conditions.