Recombinant Rat OSM

Name

Recombinant Rat OSM (Recombinant Rat Oncostatin-M; rRtOSM)；重组大鼠肿瘤抑制素

Synonyms

MGC20461; oncostatin M; oncostatin-M

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 2.0ng/ml, corresponding to a specific activity of >5.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, 0.1% Tween-80.

Endotoxin

Less than 1EU/μg of rRtOSM as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Oncostatin-M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 215 amino acids and has a molecular mass of 22.4kDa. It has approximately 48% and 72% amino acid sequence identity with human and murine OSM.

Amino Acid Sequence

KRGCSSSSPK LLSQLKSQAN ITGNTASLLE PYILHQNLNT LTLRAACTEH PVAFPSEDML RQLSKPDFLS TVHATLGRVW HQLGAFRQQF PKIQDFPELE RARQNIQGIR NNVYCMARLL HPPLEIPEPT QADSGTSRPT TTAPGIFQIK IDSCRFLWGY HRFMGSVGRV FEEWGDGSRR SRRHSPLWAW LKGDHRIRPS RSSQSAMLRS LVPR

产品编号

产品名称

包装

KL-6344-2μg

Recombinant Rat OSM

2μg

KL-6344-10μg

Recombinant Rat OSM

10μg

KL-6344-100μg

Recombinant Rat OSM

100μg

KL-6344-1mg

Recombinant Rat OSM

1mg

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说明书

1份