Recombinant Rat IL-10

Name

Recombinant Rat IL-10 (Recombinant Rat Interleukin-10; rRtIL-10)；重组大鼠白细胞介素10

Synonyms

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in 20mM Tris-HCl, pH8.0, 100mM NaCl.

Endotoxin

Less than 0.1EU/μg of rRtIL-10 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. Recombinant rat IL-10 is a 160 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 37kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 Rα/IL-10R1 chains, resulting in recruitment of two IL-10 Rβ/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10Rβ does not bind IL-10 by itself but is required for signal transduction. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.

Amino Acid Sequence

SKGHSIRGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNILLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN

产品编号

产品名称

包装

KL-6290-2μg

Recombinant Rat IL-10

2μg

KL-6290-10μg

Recombinant Rat IL-10

10μg

KL-6290-100μg

Recombinant Rat IL-10

100μg

KL-6290-1mg

Recombinant Rat IL-10

1mg

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说明书

1份