Recombinant Murine VEGF120

Name

Recombinant Murine VEGF120 (Recombinant Murine Vascular Endothelial Growth Factor120; rMuVEGF120)；Recombinant Mouse VEGF120 ; 重组小鼠血管内皮生长因子120

Synonyms

VAS; Vasculotropin; VEGFA; VPF; MVCD1; vascular endothelial growth factor A; Vascular permeability factor; VEGF-A; VEGFMGC70609; VPFvascular endothelial growth factor

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5ng/ml, corresponding to a specific activity of >2.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rMuVEGF120 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells that stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. VEGF signals through the three receptors: fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Mouse express alternately spliced isoforms of 120, 164, 182 amino acids (a.a.) in length. The VEGF120 shares 98%a.a. sequence identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively.

Amino Acid Sequence

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R

产品编号

产品名称

包装

KL-6119-2μg

Recombinant Murine VEGF120

2μg

KL-6119-10μg

Recombinant Murine VEGF120

10μg

KL-6119-100μg

Recombinant Murine VEGF120

100μg

KL-6119-1mg

Recombinant Murine VEGF120

1mg

-

说明书

1份