Recombinant Murine FGF-21

Name

Recombinant Murine FGF-21 (Recombinant Murine Fibrobalst Growth Factor-21; rMuFGF-21)；Recombinant Mouse FGF-21 ; 重组小鼠成纤维细胞生长因子21

Synonyms

FGF-21; fibroblast growth factor 21

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5μg/ml, corresponding to a specific activity of >2.0×10³IU/mg in the presence of 5μg/ml of rMuKlotho-β and 10μg/ml of heparin.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 3X PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rMuFGF-21 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors. Recombinant murine FGF-21 contains 182 amino acids residues and show limited binding to heparin. In addition, Murine FGF-21 respectively shows 81% and 92%a.a. identity to human and rat FGF-21, and it show activity on human and rat cells.

Amino Acid Sequence

AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS

产品编号

产品名称

包装

KL-6101-5μg

Recombinant Murine FGF-21

5μg

KL-6101-25μg

Recombinant Murine FGF-21

25μg

KL-6101-100μg

Recombinant Murine FGF-21

100μg

KL-6101-1mg

Recombinant Murine FGF-21

1mg

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说明书

1份