CD256; TALL2; TNFSF13; TRDL1; ZTNF2; A proliferation-inducing ligand; APRILFLJ57090; CD256; CD256 antigen; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using activated T cells.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 0.02% Tween-20.
Endotoxin
Less than 0.1EU/μg of rMusAPRIL/TNFSF13 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
A proliferation-inducing ligand (APRIL), also known as TALL-2 and TRDL-1, is a type II transmembrane protein that is a member of the TNF ligand superfamily (TNFSF13). APRIL expression is low in normal tissues, but is elevated in several types of tumors and transformed cell lines. APRIL stimulates proliferation of tumor cell lines and increases tumorigenicity in nude mice. Recombinant murine soluble APRIL is a 16.4kDa protein, consisting of 146 amino acid residues.