Recombinant Murine IL-21

Name

Recombinant Murine IL-21 (Recombinant Murine Interleukin-21; rMuIL-21)；Recombinant Mouse IL-21 ; 重组小鼠白细胞介素21

Synonyms

IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 T cells is less than 25ng/ml, corresponding to a specific activity of >4.0×10⁴IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4.

Endotoxin

Less than 0.1EU/μg of rMuIL-21 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

IL-21 is encoded by IL-21 gene located on Chr. 4 in mouse. It is a pleiotropic cytokine produced by CD4⁺ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. The murine IL-21 precursor contains a predicted 17 amino acid (a.a.) signal sequence and a 129a.a. mature chain. Mature murine IL-21 shares 66%, 59%, 58% and 88%a.a. sequence identity with mature canine, human, rabbit and rat IL-21, respectively.

Amino Acid Sequence

HKSSPQGPDR LLIRLRHLID IVEQLKIYEN DLDPELLSAP QDVKGHCEHA AFACFQKAKL KPSNPGNNKT FIIDLVAQLR RRLPARRGGK KQKHIAKCPS CDSYEKRTPK EFLERLKWLL QKMIHQHLS

产品编号

产品名称

包装

KL-5957-2μg

Recombinant Murine IL-21

2μg

KL-5957-10μg

Recombinant Murine IL-21

10μg

KL-5957-100μg

Recombinant Murine IL-21

100μg

KL-5957-1mg

Recombinant Murine IL-21

1mg

-

说明书

1份