Recombinant Human BD-2

Name

Recombinant Human BD-2 (Recombinant Human β-defensin 2; rHuBD-2)；重组人β-防御素2

Synonyms

BD-2; DEFB2; DEFB4A; DEFB4B; HBD-2; SAP-1; Beta-defensin 2; DEFB-2; DEFB2DEFB102; DEFB4beta-defensin 4A; Defensin, beta 2Skin-antimicrobial peptide 1; defensin, beta 4; defensin, beta 4A; HBD-2; hBD-2; SAP1BD-2

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration range of 10-100ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 130mM NaCl.

Endotoxin

Less than 1EU/μg of rHuBD-2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca²⁺ and Mg²⁺ ions. Furthermore, they can affect the stability of the membrane.

Amino Acid Sequence

GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P

产品编号

产品名称

包装

KL-5804-5μg

Recombinant Human BD-2

5μg

KL-5804-20μg

Recombinant Human BD-2

20μg

KL-5804-100μg

Recombinant Human BD-2

100μg

KL-5804-1mg

Recombinant Human BD-2

1mg

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说明书

1份