Recombinant Human FGF-21

Name

Recombinant Human FGF-21 (Recombinant Human Fibroblast Growth Factor-21; rHuFGF-21)；重组人成纤维细胞生长因子21

Synonyms

FGF-21; fibroblast growth factor 21

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5μg/ml, corresponding to a specific activity of >2.0×10³IU/mg in the presence of 5μg/ml of rMuKlotho-β and 10μg/ml of heparin.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuFGF-21 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene and it is specifically induced by HMGCS2 activity. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure and they are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21 contains a 28a.a. signal sequence and a 181a.a. mature region but show limited binding to heparin. In addition, Mature human FGF-21 respectively shows 81%a.a. identity to murine and rat FGF-21, and is known to be active on murine cells.

Amino Acid Sequence

HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S

产品编号

产品名称

包装

KL-5493-5μg

Recombinant Human FGF-21

5μg

KL-5493-25μg

Recombinant Human FGF-21

25μg

KL-5493-100μg

Recombinant Human FGF-21

100μg

KL-493-1mg

Recombinant Human FGF-21

1mg

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说明书

1份