Recombinant Human Epigen

Name

Recombinant Human Epigen (Recombinant Human Epigen; rHuEpigen)；重组人Epigen

Synonyms

EPGN

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300ng/ml, corresponding to a specific activity of >3.3×10³IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuEpigen as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Epigen is a member of the epidermal growth factor family and is expressed by several tissues, including the testis, liver, heart and in certain tumor cells. It is strongly mitogenic for fibroblasts and epithelial cells, despite its relatively weak affinity for its main receptor, ErbB1. The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated depletion. The gene of human Epigen encodes a 154 amino acid (a.a.) residue type I transmembrane precursor glycoprotein which contains a 22a.a. signal peptide, an 88a.a. extracellular domain, a 21a.a. transmembrane domain and a 23a.a. cytoplasmic domain. By alternative splicing of the gene, Epigen has several isoforms. The mature, shed form of human Epigen (54-104a.a.) shares 92%, 94% and 94%a.a. sequence identity with mouse, rat and equine Epigen, respectively.

Amino Acid Sequence

AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA

产品编号

产品名称

包装

KL-5565-5μg

Recombinant Human Epigen

5μg

KL-5565-25μg

Recombinant Human Epigen

25μg

KL--5565-100μg

Recombinant Human Epigen

100μg

KL-5565-1mg

Recombinant Human Epigen

1mg

-

说明书

1份