Recombinant Human IFN-α1b (Recombinant Human Interferon-α1b; rHuIFN-α1b);重组人干扰素α1b
Synonyms
IFL; IFN; IFNA; IFNA13; IFN-ALPHA; IFN-alphaD
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0×108IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4, containing 4% mannitol and 1% HSA.
Endotoxin
Less than 1EU/μg of rHuIFN-α1b as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Interferon alpha-1, also named interferon alpha-D, is belonging to the type I interferon family and is encoded by the IFNA1gene in humans. Interferon alpha-1 is expressed in peripheral blood leukocytes and lymphoblastoid cells. IFNα-1 contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. It plays an important role in inducing non-specific resistance against a broad range of viral infections, and also affects cell proliferation and modulates immune responses. IFNα-1a and IFNα-1b are two forms of interferon alpha-1 except for the substitution of alanine for valine at position 137. Interferon-alpha represents a group of related but distinct proteins that share over 95% amino acid sequence homology.